Figure 6.

HDAC6 coordinates with CLIP-170 to regulate pancreatic cancer cell migration. (A) PANC-1 cells transfected with HDAC6 siRNA and GFP, GFP-HDAC6, or GFP-CLIP-170 (full-length or various truncated forms) for 48 h were scratched, and wound margins were imaged 0 h and 30 h later. (B) Experiments were performed as in (A), and the extent of wound closure was quantified. (C) Cells were transfected as in (A), and transwell migration experiments were performed and cells migrated to the underside of the insert were stained with crystal violet. (D) Experiments were performed as in (C), and the amount of migrated cells was quantified