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Silencing of GLS increases intracellular level of Gln in T98G (a) and TGAB (b) cells. T98G and TGAB cells were transfected with siGls3–7. The level of intracellular Gln was measured at 48 h post-transfection. The results are mean ± SD for four independent determinations for each cell line. *p < 0.05 vs untreated cells and treated with scrambled siRNA (scr3); **p < 0.05 vs T98G counterparts as tested with one-way ANOVA followed by Tukey’s test
