Fig. 2.
The effects of Hpa110–42 expression on English grain aphid colonization and reproduction on wheat leaves. (A, B) Uniform 10-day-old adults of apterous and agamic aphid females were placed on the upper sides of the top two expanded leaves (10 aphids/leaf) of 30-day-old plants. Leaf colonies were surveyed 24h later. A total of 1200 aphids were monitored in four experimental repetitions (each containing 30 plants). The numerical values are means ±SDs, and different letters on the SD bars indicate significant differences by one-tailed ANOVA and LSD test (P<0.01). (A) Values of plant colonization preference were scored as the number of aphids that stayed in their colonies on leaves. The percentage decrease in the value of preference for a Y16:Hpa110–42 line was calculated in comparison with the value of preference for Y16. (B) The reproduction rate is given as the ratio between the total number of newborn nymphs and the total number of adults on leaf colonies. The percentage decrease in the rate of reproduction on leaves of a Y16:Hpa110–42 line was calculated in comparison with the rate of reproduction on Y16 leaves. (This figure is available in colour at JXB online.)