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. 2014 Jan 30;65(6):1455–1465. doi: 10.1093/jxb/eru003

Fig. 4.

Fig. 4.

HOMEOBOX and cytokinin pathway genes are regulated by class I BPCs. (A) ChIP analysis revealing that class I BPCs directly bind the GA-rich sites in the promoter of different HOMEOBOX transcription factors; the bpc1-2 bpc2 bpc3 triple mutant was used as a negative control. Each bar shows the average of three independent ChIP experiments (±standard deviation). (B) Expression analyses of IPT7 in wild-type and bpc1-2 bpc2 bpc3 young inflorescences. (C, D) In situ hybridization with ARR7-specific antisense probe using wild-type (C) and bpc1-2 bpc2 bpc3 (D) inflorescences. A stronger and more diffuse signal was detectable in the mutant IM (arrowhead), FM, pedicels, and stem (arrow). (E) ChIP analysis revealing that class I BPCs directly bind the GA-rich site in the ARR7 promoter; the bpc1-2 bpc2 bpc3 triple mutant was used as a negative control. Each bar shows the average of three independent ChIP experiments (±standard deviation). Pd, pedicel; Sm, stem. Bars, 50 µm. (This figure is available in colour at JXB online.)