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. 2014 Feb 5;65(6):1497–1512. doi: 10.1093/jxb/eru014

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© The Author 2014. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 10. — Secretion of acid phosphatases and organic acids was reduced in NR23 under phosphate-deficient conditions. (A) A root from each seedling of Col-0, NR23, NR23-related lines (#2-1 and #6-9), and complementation lines of #6-9 (com1 and com2) was submerged in 2ml of the reaction medium for 9min as described in the Materials and methods. It was confirmed that the activity was completely inhibited by molybdate at 1mM (+Mo), indicating that the detected activity was of the acid phosphatase. Values are means ±SD (n=5). *P<0.05. (B) Secretion of organic acids was reduced in NR23 under Pi-deficient conditions. Roots of 21-day-old seedlings were submerged in water for 3h and then aliquots of the water were used for quantification of malate and citrate. Values are means ±SD (n=5). ***P<0.005.