Fig. 3.

Growth of NR23 was poor under mineral-deficient conditions. Col-0 and NR23 were grown under sterile conditions on Hoagland medium containing 0.5% sucrose in vertical plates at 22 °C under long-day conditions. Deficiencies of Cu, Ca, N, K, P, and Zn were assayed using Ina agar, which contained low concentrations of a specific element (A, B, E). Nacalai agar was used for analyses of Fe, Mn, B, and Mg (C, D, F). Plants were subjected to measurements of fresh weight (A, C, E) and primary root length (B, D) at 14 d (A–D, F) or 26 d (E). Values are means ±SD; n≥30 (A–D), n=100 (E). Significant differences from Col-0 are indicated by asterisks (*P<0.05; **P<0.01, ***P<0.005). Typical seedlings grown under iron-deficient conditions were photographed (F).