Figure 8.

Boxplot of recovery time when (a) the first movement and (b) complete recovery was observed in N. denticulata individuals after anaesthesia in different MS-222 concentrations and bath durations. The horizontal line shows the median, boxes the first and third quartiles, whiskers the highest and lowest values that are within the 1.5 inter-quartile range, black dots the outliers and numbers above boxes the sample size. ^a A ten-minute bath duration is insufficient to induce anaesthesia at a concentration of 1500 mg L⁻¹. ^b A thirty-minute bath duration is lethal to some individuals at a concentration of 2000 mg L⁻¹ and was not performed for higher concentrations. ^c The forty minute bath duration was not performed for concentrations higher than 1500 mg L⁻¹. ^d Some individuals that showed signs of movement were not fully recovered at the end of the assayed period.