Table 1.
Primers | Sequence (5′–3′) | Restriction Site |
---|---|---|
rfaD-U-F | CCGCTCGAGTCCGTTACACCTTCAGCA | XhoI |
rfaD-U-R | CGGAATTCGTGGCTGATGTGAATCTGTGGT | EcoRI |
rfaD-D-F | CCCAAGCTTTACTTGCCGTCCCACTCG | HindIII |
rfaD-D-R | AAAACTGCAGGCTTTATCGGCAGCAACA | PstI |
kan-FRT-F | CGGAATTCGTGTAGGCTGGAGCTGCTTCG | EcoRI |
kan-FRT-R | CCCAAGCTTGCCATTAATTCACTGATCAG | HindIII |