Table 2.
Bacterial strains and plasmids used in this study.
| Strains or Plasmids | Description | Source |
|---|---|---|
| Strains | ||
| W3110 | Wild-type E. coli, F−, λ− | Laboratory strain |
| W3110/pKD46 | W3110 transformed by pKD46 | Laboratory strain |
| WBB06 | W3110 mutant with a mutation of waaC and waaF genes (rfaC-rfaF::tet6) | [22] |
| WJW00 | W3110 ΔrfaD | This Study |
| Plasmids | ||
| pBluscript II SK+ | Cloning vector, ColE1, lacZ, AmpR | Stratagene |
| pBS-D-Fkan | Plasmid for deleting rfaD in E. coli | This Study |
| pKD46 | ParaBγβ exo, Repts, AmpR | [23] |
| pKD13 | oriR6K, FRT KanR FRT, AmpR | [23] |
| pCP20 | FLP+, λ cI857, λpRRepts, CamR, AmpR | [24] |