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. 2014 Mar 13;12(3):1530–1544. doi: 10.3390/md12031530

Figure 5.

Figure 5

Effect of CS5931 on the expression of VEGF protein and mRNA. Cells were treated without (1) or with 10 (2), 20 (3), 30 (4) and 40 μg/mL (5) of CS5931, respectively. After incubation for 24 h, the VEGF expression was analyzed by Western Blotting (A) and RT-PCR analysis (C). (B) and (D) represent the quantitative evaluation of the expression of VEGF. Results are normalized to untreated cells. All experiments were repeated more than three times. Values represent the means ± SD of triplicate measurements. * P < 0.05, ** P < 0.01 versus medium control.