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. 1971 Apr;47(4):576–580. doi: 10.1104/pp.47.4.576

An Evaluation of 4-S-Methyl-2-Keto-Butyric Acid as an Intermediate in the Biosynthesis of Ethylene

Morris Lieberman 1, A T Kunishi 1
PMCID: PMC396729  PMID: 16657663

Abstract

Stimulation of ethylene production by cauliflower (Brassica oleracea var. botrytis L.) tissue in buffer solution containing 4-S-methyl-2-keto-butyric acid is not due to activation of the natural in vivo system. Increased ethylene production derives from an extra-cellular ethylene-forming system, catalyzed by peroxidase and other factors, which leak from the cauliflower tissue and cause the degradation of 4-S-methyl-2-keto-butyric acid. This exogenous ethylene-forming system is similar to the ethylene-forming horseradish peroxidase system which utilizes methional or 4-S-methyl-2-keto-butyric acid as substrate. We conclude that 4-S-methyl-2-keto-butyric acid is probably not an intermediate in the biosynthetic pathway between methionine and ethylene.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baur A. H., Yang S. F. Precursors of ethylene. Plant Physiol. 1969 Sep;44(9):1347–1349. doi: 10.1104/pp.44.9.1347. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Burg S. P., Clagett C. O. Conversion of methionine to ethylene in vegetative tissue and fruits. Biochem Biophys Res Commun. 1967 Apr 20;27(2):125–130. doi: 10.1016/s0006-291x(67)80050-0. [DOI] [PubMed] [Google Scholar]
  3. Lieberman M., Kunishi A. T. Stimulation of ethylene production in tomato tissue by propionic Acid. Plant Physiol. 1969 Oct;44(10):1446–1450. doi: 10.1104/pp.44.10.1446. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Lieberman M., Kunishi A. Stimulation of ethylene production in apple tissue slices by methionine. Plant Physiol. 1966 Mar;41(3):376–382. doi: 10.1104/pp.41.3.376. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Mapson L. W., March J. F., Wardale D. A. Biosynthesis of ethylene. 4-methylmercapto-2-oxobutyric acid: an intermediate in the formation from methionine. Biochem J. 1969 Dec;115(4):653–661. doi: 10.1042/bj1150653. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Mapson L. W., Mead A. Biosynthesis of ethylene. Dual nature of cofactor required for the enzymic production of ethylene from methional. Biochem J. 1968 Aug;108(5):875–881. doi: 10.1042/bj1080875. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Mapson L. W., Wardale D. A. Biosynthesis of ethylene. Enzymes involved in its formation from methional. Biochem J. 1968 Apr;107(3):433–442. doi: 10.1042/bj1070433. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Mapson L. W., Wardale D. A. Biosynthesis of ethylene. Formation of ethylene from methional by a cell-free enzyme system from cauliflower florets. Biochem J. 1967 Feb;102(2):574–585. doi: 10.1042/bj1020574. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Takeo T., Lieberman M. 3-methylthiopropionaldehyde peroxidase from apples: an ethylene-forming enzyme. Biochim Biophys Acta. 1969 Apr 22;178(2):235–247. doi: 10.1016/0005-2744(69)90393-3. [DOI] [PubMed] [Google Scholar]
  10. Yang S. F. Biosynthesis of ethylene. Ethylene formation from methional by horseradish peroxidase. Arch Biochem Biophys. 1967 Nov;122(2):481–487. doi: 10.1016/0003-9861(67)90222-6. [DOI] [PubMed] [Google Scholar]
  11. Yang S. F. Further studies on ethylene formation from alpha-keto-gamma-methylthiobutyric acid or beta-methylthiopropionaldehyde by peroxidase in the presence of sulfite and oxygen. J Biol Chem. 1969 Aug 25;244(16):4360–4365. [PubMed] [Google Scholar]

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