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PCR of sortase gene from S. mutans NG8, and the sortase-defective and sortase-complemented strains. Lanes 1–3, wild-type NG8; lanes 4–6, sortase-defective; and lanes 7–9, sortase-complemented. Each lane represents cells from a different experimental culture. The arrowhead (far left) indicates the location of the 741 bp sortase gene and the markers (far right) indicate the range of 600–1 000 bp. PCR, polymerase chain reaction.
