Table 1. Specific primers used for real-time PCR.
Primers | Sequences |
---|---|
16S rRNA | 5′-AGCGTTGTCCGGATTTATTG-3′ |
5′-CTACGCATTTCACCGCTACA-3′ | |
gtfB | 5′-CACTATCGGCGGTTACGAAT-3′ |
5′-CAATTTGGAGCAAGTCAGCA-3′ | |
gtfC | 5′-GATGCTGCAAACTTCGAACA-3′ |
5′-TATTGACGCTGCGTTTCTTG-3′ | |
gtfD | 5′-TTGACGGTGTTCGTGTTGAT-3′ |
5′-AAAGCGATAGGCGCAGTTTA-3′ | |
gbpC | 5′-AATTCTGATACTGTTGCAGCACCTA-3′ |
5′-TTCTGTTGCAGCCGGTTCT-3′ | |
antigen I/II | 5′-GGATCTGGCTGGGATAGTTCAG-3′ |
5′-GACCAGACATGCGGATAGCA-3′ | |
dexA | 5′-GACAACTGCGGCCATTGC-3′ |
5′-CCAGAGCAGCCAGAGACATG-3′ |
Gbp, glucan-binding protein; GTF, glucosyltransferases; PCR, polymerase chain reaction.