Table 1. Minimum lipid concentrations required to inhibit or kill P. gingivalis. Pairwise comparisons showed that all MIC and MBC values for lipid treatments were significantly different from each other (5% level of statistical significance).
| Compounds | Meana±s.d. (median) of concentration/(µg⋅mL−1) | |
|---|---|---|
| MIC | MBC | |
| Sphingosine | 0.2±0.8 (0.2) | 0.3±0.0 (0.3) |
| Phytosphingosine | 0.8±0.3 (0.8) | 1.0±0.0 (1.0) |
| Dihydrosphingosine | 0.4±0.2 (0.4) | 0.5±0.2 (0.6) |
| Sapienic acid | 58.6±11.0 (58.6) | 62.5±0.0 (62.5) |
| Lauric acid | 125.0±0.0 (125.0) | 218.8±57.9 (250.0) |
| SMAP-28b | 5.0±0.0 (5.0) | 20.0±0.0 (20.0) |
| Chlorhexidine | 3.9±0.0 (0.0) | 7.8±0.0 (0.0) |
MBC, minimum bactericidal concentration; MIC, minimum inhibitory concentration.
a
Mean±standard deviation (median); n=8 per lipid treatment.
b
SMAP28 and chlorhexidine were used as positive controls to show that the microdilution assays were set up properly and MICs/MBCs were accurate and within previously reported ranges; SMAP28 and chlorhexidine results were not included in the statistical analysis.