Figure 4. Kinesin-2 heterodimers exhibit different translocation preferences.

Distribution of truncated KIF3s following co-expression with their heterodimer-forming partners. (A) When expressed together, both KIF3A⁴¹⁹-tdTomato and KIF3B⁴¹⁴-YFP accumulated at axonal tips; (B) co-expression of KIF3A⁴¹⁹-tdTomato and KIF3C⁴⁴¹-GFP resulted in labeling at axonal tips and some dendritic tips. Line scans of the boxed regions confirm strong enrichment of KIF3A/KIF3C heterodimers at dendritic tips. Scale bars= 20μm. (C) Translocation preferences of truncated KIF3A/3B and KIF3A/3C heterodimers, based on the analysis of 67 or 51 cells quantified as in Fig. 2. Data points for KIF1A³⁹³ and KIF5C⁵⁵⁹ were taken from the experiment shown in Figure 2I. Images in A and B were assembled from four microscope fields as described in Materials and Methods.