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. Author manuscript; available in PMC: 2014 Mar 27.

Published in final edited form as: Traffic. 2012 Jan 24;13(4):549–564. doi: 10.1111/j.1600-0854.2011.01325.x

Truncated KIF1B (A) and KIF21B (D) accumulated at all neurite tips whereas truncated KIF17 (B) only accumulated at a subset of neurite tips. The remaining kinesins failed to accumulate at any tips. For KIF13-Fv constructs, cells were incubated with 10nM AP21087 for 4–6h before imaging. (I) KIF21A,, which is unable to accumulate in immature neurons, translocated to neurite tips following Taxol treatment (10 nM) for 1 h. Line scans of the average intensity along the neurite in the boxed region (below) confirm the redistribution of KIF21A⁴¹⁷-GFP. (J) The accumulation of KIF21A and KIF5C but not Kinesin-2s in Stage 2 cells was enhanced by 10nM Taxol treatment for 4h. The figure shows the percentage of cells with truncated kinesin accumulation in at least one neurite; values are means and SEMs from 3 independent experiments. Dissociated hippocampal neurons were co-electroporated with tdTomato and a truncated kinesin or with a pair of truncated KIF3s. Scale bar: 20μm.

Truncated KIF1B (A) and KIF21B (D) accumulated at all neurite tips whereas truncated KIF17 (B) only accumulated at a subset of neurite tips. The remaining kinesins failed to accumulate at any tips. For KIF13-Fv constructs, cells were incubated with 10nM AP21087 for 4–6h before imaging. (I) KIF21A,, which is unable to accumulate in immature neurons, translocated to neurite tips following Taxol treatment (10 nM) for 1 h. Line scans of the average intensity along the neurite in the boxed region (below) confirm the redistribution of KIF21A⁴¹⁷-GFP. (J) The accumulation of KIF21A and KIF5C but not Kinesin-2s in Stage 2 cells was enhanced by 10nM Taxol treatment for 4h. The figure shows the percentage of cells with truncated kinesin accumulation in at least one neurite; values are means and SEMs from 3 independent experiments. Dissociated hippocampal neurons were co-electroporated with tdTomato and a truncated kinesin or with a pair of truncated KIF3s. Scale bar: 20μm.