Table II.

Kinesin Constructs

a. Wild- type truncation constructs:
Kinesin	Amino acids	FP tag	Reference
KIF5A	1–559	eGFP	NP 032473
KIF5B	1–555	YFP	NP 032474
KIF5C	1–388, 1–559	eGFP	NP 001101200
KIF3A	1–419	tdTomato	NP 032469
KIF3B	1–414	YFP	NP 032470
KIF3C	1–441	eGFP	NP 032471
KIF17	1–511	YFP	NP 034753
KIF1A	1–393	tdTomato	XP 343631
KIF1B	1–386	tagRFP	NP 476548
KIF1C	1–389	eGFP	NP 006603
KIF13A	1–392, 1–435, 1–565, 1–639	eGFP	NP 034747
KIF13B	1–393, 1–436, 1–568, 1–648	eGFP	NP 001074646
KIF21A	1–417	eGFP	NP 001102511
KIF21B	1–409	eGFP	NP 001034561

b. Chimeras with interchanged microtubule-binding regions
Name	Deleted region	Inserted region	FP tag
KIF5C559L81A	KIF5C142–176	KIF1A153–187	eGFP
KIF5C559L111A	KIF5C238–253	KIF1A254–269	eGFP
KIF5C559L121A	KIF5C272–275	KIF1A288–302	eGFP
KIF1A393L85C	KIF1A153–187	KIF5C142–176	tdTomato
KIF1A393L115C	KIF1A254–269	KIF5C238–253	tdTomato
KIF1A393L125C	KIF1A288–302	KIF5C272–275	tdTomato
KIF3B414L113C	KIF3B253–262	KIF3C254–289	YFP
KIF3C441L113B	KIF3C254–289	KIF3B253–262	eGFP

c. Chimeras with interchanged dimerization domains
Name	N-terminal (motor domain)	C-terminal (dime- rization domain)	FP tag
KIF3AMD3BHD	KIF3A1–361	KIF3B357–414	YFP
KIF3AMD3CHD	KIF3A1–357	KIF3C380–441	eGFP
KIF3BMD3AHD	KIF3B1–356	KIF3A362–419	tdTomato
KIF3BMD3CHD	KIF3B1–352	KIF3C380–441	eGFP
KIF3CMD3AHD	KIF3C1–379	KIF3A358–419	tdTomato
KIF3CMD3BHD	KIF3C1–379	KIF3B353–414	YFP
KIF1AMD5C559	KIF1A1–352	KIF5C326–559	tdTomato