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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: J Immunol. 2014 Mar 3;192(7):3156–3165. doi: 10.4049/jimmunol.1301726

Figure 4. Neontal neutrophils and macrophages have decreased ROS production compared to adult mice.

Figure 4

A. Neonatal mouse neutrophils (CD11b+, Ly6G+) and macrophages (CD11b+, Ly6G−, F480+) one day after sepsis had decreased ability to produce reactive oxygen species (ROS) in response to PMA stimulation compared to adult mice one day after sepsis (p<0.0001, 2-way ANOVA). B. Functional pathway analysis showed that elderly mice had increased up regulation of genes involved in the synthesis, production, and metabolism of ROS compared to neonatal and/or young adult mice, which is consistent with in vivo results. Heatmaps show the of gene expression of the functional category “Free Radical Scavenging” 2 hours after sepsis in neonatal, young adult, and elderly mice. Orange represents pathways with an over-expression of genes leading to the activation of the pathway and white represents genes that are neither up or down regulated to significance level of p<0.001. Graph shows the various subcategories and their corresponding significance level (Z-score) of up or down regulation.