Figure 4.
Effects of ceramide analogs on breast cancer intrinsic cell death. MCF-7TN-R cells were treated with double IC50 concentrations (the IC50 values determined from MTT viability assay) for 24 h. (A) Treatment with analog 406 induced a 4.30 ± 1.10 fold (*p <0.05) increase in apoptosis compared to vehicle control. (B) Treatment with analog 406 induced a 3.59 ± 0.45 fold (*p <0.05) increase in caspase-9 activity compared to vehicle control. DMSO, vehicle control; Taxol and C8-Cer, positive control. The values are the mean ± SE of three independent experiments.