. Author manuscript; available in PMC: 2015 Feb 15.

Published in final edited form as: Bioorg Med Chem. 2014 Jan 8;22(4):1412–1420. doi: 10.1016/j.bmc.2013.12.065

Table 2.

IC₅₀ values of ceramide analogs in clonogenic survival assay (μM)

Compound	MCF-7	MDA-MB-231	MCF-7TN-R
	IC₅₀ (μM)
401	5.07 ± 0.17	1.45 ± 0.06	1.85 ± 1.22
402	5.69 ± 0.65	3.17 ± 0.10	5.19 ± 0.11
403	4.18 ± 0.52	1.59 ± 0.10	5.62 ± 0.44
404	10.16 ± 0.14	17.55 ± 0.10	10.05 ± 0.59
406	3.40 ± 0.37	1.40 ± 0.13	1.81 ± 1.18

MCF-7, MDA-MB-231, and MCF-7TN-R cells were treated with increasing concentrations of the analogs studied and allowed to grow until colony formation was noted (generally 10–12 days). The values are the mean ± SE of three independent experiments.