Fig. 6.

BrdU-labeled nuclei in polyp and in tissue ball formed in 2 days primary cell culture. Semi-thin (1 μm) sections stained with toluidine blue and basic fuchsin of a polyp and b TB. Insets are light micrographs indicating the orientation of the sections in respectively the P. damicornis polyp or the TB. Fluorescence of nuclei stained with DAPI (in blue) (c, d), immunolabeled with Alexa-594 (in red) (e, f) and their image overlay (g, h) in semi-thin sections of a polyp (a, c, e, g) and a tissue ball (b, d, f, h). sk. skeleton, pse. pseudo-stratified epithelium, o.g. oral gastroderm, ab.g. aboral gastroderm, cal. calicoderm, g.c. gastric cavity. Scale bars: 100 μm