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. 2013 Jan 17;65(5):839–850. doi: 10.1007/s10616-012-9524-4

Table 1.

The gene and sequence of forward (F) and reverse (R) primers used in the real-time PCR reactions

Gene Primer References or no of NCBI
GAPDH F:5′ACAAGATTGTGAAGG TCG GTG TCA 3′
R:5′AGCTTCCCATTCTCAGCCTTGACT 3′
Sugaya et al. (2005) (with modification)
CASP8 F:5′ GCAAAAGCACGGGAGAAAGT 3′
R:5′ TGCATCCAAGTGTGTTCCATT3′
Castaneda and Rosin-Steiner (2006)
CASP9 F:5′ GCTCTTCCTTTGTTCATCTCC 3′
R:5′ GTTTTCTAGGGTTGGCTTCG 3′
Chen et al. (2009)
BIRC5 F:5′ AGCCCTTTCTCAAGGACCAC 3′
R:5′ TGGCTCGTTCTCAGTGGGGCAGT 3′
Zhang et at. (2001) (with modification)
BCL-XL F:5′ TGGGCTCACTCTTCAGTCGGAAAT 3′
R:5′ ATGTAG TGGTTCTCCTGGTGGCAA 3′
NCBI ID: NM_138578.1
(designera)

aSequence of forward and reverse primers was designed according to IDT software http://www.idtdna.com/Scitools/Applications/Primerquest