Fig. 3.

Dot plots and histograms representing a standard gating protocol. a Gates around lymphocytes and monocytes on an FSC versus SSC dot plot. b Gates around CD3⁺ and CD14⁺ cells on an APC CD3 versus FITC CD14 dot plot. c Gates around CD3⁺ and CD19⁺ cells on an APC CD3 versus PE-Cy7 CD19 dot plot. d Statistical analysis. e Histogram of IL-1RII PE from CD3⁺ T lymphocytes. f Histogram of IL-1RII PE from CD14⁺ monocytes. g Histogram of IL-1RII PE from CD19⁺ B lymphocytes. Interval gates were adjusted using isotype control sample