Figure 1.

Schematic drawings of the domain structure of recombinant matrilins variants (a) and analysis recombinant matrilin products (b). The recombinant proteins of matrilins were separated on a 4–15% gel, blotted to a membrane, and incubated with antiserum matrilin-2 antibody (upper pattern, both long and short forms of matrilin-2); FLAG tag ((b) middle pattern, long, short, and deleted matrilin-2); V5 tag (bottom pattern, all forms of matrilin-2, matrilin-1, and matrilin-3). The Western blot results showed our antibody can detect two different isoforms of matrilin-2 ((b) lanes 1 and 2 upper). If genetic engineering deletion unique domain mutant of mini matrilin-2, the recombinant product would not be detectable by our matrilin-2 antibody ((b) lane 3 upper); this product can be recognized by tags ((b) FLAG tag, lane 3 middle, and V5 tag, lane 3 bottom). This antibody is no cross reaction with other matrilins, such as matrilin-1 ((b) lane 4) and matrilin-3 ((b) lane 5).