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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: Anal Biochem. 2014 Jan 3;450:20–26. doi: 10.1016/j.ab.2013.12.031

Table 1.

Kinetic parameters for decarboxylation of OMP catalyzed by wild-type EcOMPDC, wild-type ScOMPDC, and Q215A/R235A mutant ScOMPDCa

Kinetic parameter Wild-type EcOMPDCb Wild-type ScOMPDCc Q215A/R235A
ScOMPDCd
kcat (s−1) 14 15 0.02
Km (µM) 22 1.4 500
kcat/Km (M−1 s−1) 6.3 · 106 1.1 · 107 13
a

At pH 7.1 (10–30 mM MOPS), 25°C, I=0.105 (NaCl) and monitored by UV spectroscopy.

b

Data from reference 5.

c

Data from reference 7.

d

Data from reference 8.