Figure 3.

IgG isotype and T cell cytokine profile induced in mice following immunization with rE7m mixed with lipo-Nter. Wild-type C57BL/6 mice were subcutaneously administered rE7m (30 μg) mixed with/without lipo-Nter (30 μg/100 μL) or PBS twice at a 2-week interval. (a) After 2 weeks, serum samples were collected, and anti-rE7m antibody titers were determined by sandwich ELISA. (b) On day 7 after the second immunization, splenocytes from immunized mice were stimulated with rE7m (100 nM), and the levels of IL-2, IFN-γ, and IL-5 in the culture supernatant were measured by ELISA. The data are expressed as the means + S.D. of the samples.