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. Author manuscript; available in PMC: 2014 Nov 4.
Published in final edited form as: Mol Pharm. 2013 Sep 30;10(11):4120–4135. doi: 10.1021/mp400115y

Figure 8.

Figure 8

Colocalization of polyplexes with Rab 5 proteins, a marker of early endosomes, at time points of 4, 8, 12, and 24 hours. Images A-D show confocal images of HeLa cells showing polyplex colocalization (monitored by imaging Cy5-pDNA) with Rab 5 protein for early endosomes at 4, 8, 12 and 24 hour timepoints for polyplexes formed with Cy5-pDNA and (A) jetPEI™, (B) Glycofect™, (C) Tr455 and (D) Tr477. The nucleus is pseudo colored yellow, the polyplexes (monitored by imaging Cy5-pDNA) are pseudo colored blue, and Rab 5 is pseudo colored in red. The z-axis is directed into the plane of image, which is shown in the x-y plane. The parameter measured was 3D colocalization of the polyplex/pDNA with Rab 5 (early endosome marker in arbitrary units), which was determined by the total of overlapping volumes, rendered from the fluorescence, between the two channels.