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Dynamic light scattering study to monitor complexation of siRNA and each of the polymers at N/P=50 in RNase-free water at 25 °C. Nanoparticle size (hydrodynamic diameter) was measured at the indicated timepoints from 0 min to 60 min. Zeta potential (triangles) was measured after the 60 min timepoint. All the experiments were completed in triplicate. It should be noted here that both T4 and Glycofect did not form polyplexes with siRNA according to DLS.
