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. Author manuscript; available in PMC: 2014 Mar 27.
Published in final edited form as: Liver Int. 2011 Mar 21;31(7):950–963. doi: 10.1111/j.1478-3231.2011.02514.x

Fig. 3.

Fig. 3

Isolation and characterization of dendritic cells (DCs) from the spleen following expansion by hydrodynamic delivery of human Fms-like tyrosine kinase receptor 3 ligand (hFlt3L) encoding plasmid. Flow cytometry analysis of splenocytes isolated from Fischer (A) or Long–Evans (LE)rats (C) before and after hFlt3L plasmid administration. Flow cytometry analysis of DCs purified from Fischer (B) and LE rats (D) by magnetic beads before or after hFlt3L plasmid administration. Thus, hydrodynamic delivery and expression of the hFlt3L plasmid greatly expands the DC population in rats. The experiment was repeated three times with similar results.