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The kinetic assays, performed at pH µM fluorogenic substrates (P1 and P2 positions are highlighted) that are specific for trypsin- and chymotrypsin/elastase-type proteases. SP activities (sensitive to inhibition by PMSF and Pefabloc SC) are expressed as relative fluorescence units (RFU/s) and normalized to the protein content of extracts. Data are displayed in a heat map.
