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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: Free Radic Biol Med. 2014 Jan 12;69:50–57. doi: 10.1016/j.freeradbiomed.2014.01.007

Table 1.

Primer sequences and PCR condition

Gene Primer sequence Ta °C Product length (bp) QPCR Amplification Efficiency* (%) n° of cycles Ref Primer Bank
SRB1 F: 5′-gaattcgcctttcgtccccg -3′
R: 5′-ttgaaggacaggctactggg3′
60.1 236 96.2 39 GenBank Accession BC112037. 1
RPL13A F: 5′-cctaagatgagcgcaagttgaa- 3′
R: 5′-ccacaggactagaacacctgctaa-3′
60.2 203 97.3 39 Pattyn et al. 2006
RPL11A F: 5′-tgcgggaacttcgcatccgc-3′
R: 5′-gggtctgccctgtgagctgc-3′
60.1 108 96.5 39 GenBank Accession NM 000975.2
GAPDH F: 5′-tgacgctggggctggcattg -3′
R: 5′-ggctggtggtccaggggtct -3′
60 134 94.6 39 GenBank Accession NM 002046.3

Data calculated by OpticonMonitor 3 Software (Bio-Rad).