Table 1.
Gene | Primer sequence | Ta °C | Product length (bp) | QPCR Amplification Efficiency* (%) | n° of cycles | Ref Primer Bank |
---|---|---|---|---|---|---|
SRB1 | F: 5′-gaattcgcctttcgtccccg -3′ R: 5′-ttgaaggacaggctactggg3′ |
60.1 | 236 | 96.2 | 39 | GenBank Accession BC112037. 1 |
RPL13A | F: 5′-cctaagatgagcgcaagttgaa- 3′ R: 5′-ccacaggactagaacacctgctaa-3′ |
60.2 | 203 | 97.3 | 39 | Pattyn et al. 2006 |
RPL11A | F: 5′-tgcgggaacttcgcatccgc-3′ R: 5′-gggtctgccctgtgagctgc-3′ |
60.1 | 108 | 96.5 | 39 | GenBank Accession NM 000975.2 |
GAPDH | F: 5′-tgacgctggggctggcattg -3′ R: 5′-ggctggtggtccaggggtct -3′ |
60 | 134 | 94.6 | 39 | GenBank Accession NM 002046.3 |
Data calculated by OpticonMonitor 3 Software (Bio-Rad).