Figure 5. Allele-specific structural differences in conformations of TCF21 3′-UTR RNAs determined by in vitro probing.

(A) Results of chemical probing of the in vitro transcribed TCF21 3′-UTR variants with varying amounts of Pb²⁺ (0, 10, 20 and 40 mM) and (B) enzymatic probing with varying amounts of RNase T1 (0, 0.25, 1 and 2 units). Major cleavage sites are shown along with their positions. miR-224 binding site is highlighted in green and rs12190287 is shown in red. (C) Summary of the major cleavage sites using either Pb²⁺ or RNase T1 and their overall cleavage strength is indicated by open and closed triangle and circles. Results are representative of at least three independent experiments.