Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Apr 1;25(7):1111–1126. doi: 10.1091/mbc.E13-12-0712

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 Joensuu et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.

PMC Copyright notice

FIGURE 7: — Myo1c actin-binding domain is crucial for proper ER phenotype in Huh-7 cells. (A) Confocal section of a live cell coexpressing Hsp47-mCherry (magenta) and EGFP- myo1cΔABL (green). Tubules (arrows) and the few remaining sheets (arrowheads) are indicated in the magnified image of the boxed area. See also Figure 5D and Supplemental Figure S2I. For rescue experiments, Huh-7 cells were transfected with Cy5-conjugated myo1c siRNA (blue) and, on the next day, siRNA-resistant construct (B) myo1c-EGFP (green) or (C) EGFP-myo1cΔABL (green) and immunolabeled for calreticulin (red). In B, tubules (arrows) and sheets/interlinked ER sheet mass (arrowheads) are indicated in the wide-field images of myo1c-depleted cell (outlined cell; D) and rescued cell (R). In C, the tubules (arrows) of nonrescued cells expressing EGFP-myo1cΔABL are indicated. See Figure 6A for comparison. Bars, 10 μm.