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. 2014 Apr 1;25(7):1137–1150. doi: 10.1091/mbc.E13-11-0689

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© 2014 Muñoz et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 2: — Rgf1p contains a functional NLS. Schematic representation of the full-length Rgf1p and several N-terminal constructs expressed as C-end GFP-fused proteins and integrated at the leu1 locus in an rgf1Δ background. The cellular locations of wild-type Rgf1p-GFP and the passenger GFP fused to N-fragments Rgf1p-N302-GFP, Rgf1p-N535-GFP, Rgf1p-302-535-GFP, and Rgf1p-302-535-NLS* were analyzed in cells grown to early log phase in YES medium.