. 2014 Apr 1;25(7):1137–1150. doi: 10.1091/mbc.E13-11-0689

TABLE 1:

S. pombe strains used in this work.

Strain	Genotype
EM28	h⁻ rgf1::nat, leu1-32, ura4D18
PG244^a	h⁻ leu1-32, ura4d18
VT14	h⁻ rgf1::his3⁺, leu1-32, ade6M210, ura4D18, his3d1
PG40	h⁻ rgf1::his3⁺, leu1-32::rgf1⁺-GFP:leu1⁺, his3d, ura4D18, ade6M210
SM209	h⁺ rgf1::his3⁺, leu1-32::rgf1⁺-GFP:leu1⁺, his3d, ura4D18, ade6M210
SM48	h⁻ rgf1::his3⁺, leu1-32::rgf1-N2∆-GFP:leu1⁺, his3d1, ura4D18, ade6M210
SM52	h⁻ rgf1::his3⁺, leu1-32::rgf1-DEP∆-GFP:leu1⁺, his3d1, ura4D18, ade6M210
SM50	h⁻ rgf1::his3⁺, leu1-32::rgf1-FPTP-GFP:leu1⁺, his3d1, ura4D18, ade6M210
SM15	h⁻ rgf1::his3⁺, leu1-32::rgf1-PH∆-GFP:leu1⁺, his3d1, ura4D18, ade6M210
SM17	h⁻ rgf1::his3⁺, leu1-32::rgf1-CNH1∆-GFP:leu1⁺, his3d1, ura4D18, ade6M210
SM99	h⁻ rgf1::his3⁺, leu1-32::rgf1302-GFP, ura4D18, ade6 M210
SM101	h⁻ rgf1::his3⁺, leu1-32::rgf1535-GFP, his3d, ura4D18, ade6 M210
EM56	h⁻ rgf1::his3⁺, leu1-32::rgf1302-535-GFP, his3d1, ura4D18, ade6 M210
SM321	h⁻ rgf1::his3⁺, leu1-32:rgf1-GFP NLS4R-4A, his3d, ura4D18, ade6M210
SM322	h⁻ rgf1::his3⁺, leu1-32::rgf1302-535-GFP-NLS4R-4A, his3d, ura4D18, ade6 M210
SM335	h⁻ rgf1::his3⁺, leu1-32::rgf1⁺-GFP his3d, ura4D18, crm1-809
SM302	h⁻ rgf1::his3⁺, leu1-32::rgf1-GFP-NES1, his3d, ura4D18, ade6M210
EM50	h⁻ rgf1::his3⁺, leu1-32::rgf1-GFP-NES2, his3d, ura4D18, ade6M210
SM330	h⁻ rad24-GFP:kan, leu1-32, ura4D18
SM382	h⁻ rad24-GFP:kan, rgf1-GST:ura4⁺, leu1-32, ura4D18
SM211	h⁻ rad24::ura4⁺, rgf1::his3⁺, leu1-32:rgf1⁺-GFP, his3d, ura4D18, ade6M210
SM317	h⁻ rad3::ura4⁺, rgf1::his3⁺, leu1-32:rgf1⁺-GFP, his3d, ura4D18
SM327	h⁻ cds1::ura4⁺, rgf1::his3⁺, leu1-32:rgf1⁺-GFP, his3d, ura4D18
SM339	h⁻ chk1::ura4⁺, rgf1::his3⁺, leu1-32:rgf1⁺-GFP, his3d, ura4D18
SM304^b	h⁻ chk1::ura4⁺, leu1-32, ura4D18
SM305^b	h⁻ cds1::ura4⁺, leu1-32, ura4D18
PG199	h⁻ rgf1::his3 his3DI leu1-32 ade6M210 ura4D18 leu1: rgf1⁺ (PTTRΔ)-GFP
SM19	h⁻ rgf1::his3⁺, leu1-32::rgf1-C189∆-GFP:leu1⁺, his3d1, ura4D18, ade6M210
EM73	h⁻ rgf1-C131∆-GFP:ura4⁺, leu1-32, ura4D18
SM394	h⁻ rgf1::his3⁺, leu1-32::rgf1-GFP-NES1, rad24::ura4⁺,his3d, ura4D18, ade6M210
SM374	h⁻ rgf1::his3⁺, leu1-32::rgf1-GFP-NES1, cds1::ura4⁺,his3d, ura4D18, ade6M210
SM616	h⁻ rgf1::nat, leu1-32::rgf1-fos9-GFP:leu1⁺, his3d1, ura4D18, ade6M210
SM624	h⁻ rgf1::nat, leu1-32::rgf1-fos6-GFP:leu1⁺, his3d1, ura4D18, ade6M210
SM620	h⁻ rgf1::nat, leu1-32::rgf1-fos3-GFP:leu1⁺, his3d1, ura4D18, ade6M210
VT183	h⁻ rgf3::ura4⁺,leu1-32::rgf3⁺-8aGFP:leu1⁺, ura4D18
SM213	h⁺ leu1-32, ura4d18 +pAL-rgf2⁺
SM680	h⁻ rad24-GST:kan, rgf1::nat, leu1-32::rgf1⁺-GFP
SM682	h⁻ rad24-GST:kan, rgf1::nat, leu1-32::rgf1-fos9-GFP:leu1⁺, his3d1, ura4D18
SM308^b	h⁻ rad22-YFP:kan, leu1-32, ura4D18
SM324	h⁻ rad22-YFP:kan, rgf1::his3⁺, leu1-32, ura4D18
SM720^a	h⁻ leu1-32 ura4D-18 rho1-596:NatMX6

All strains were generated in this study, except as otherwise noted. For two-hybrid assays we used S. cerevisiae AH109 from Clontech (Takara, Mountain View, CA).

^aPilar Perez (Instituto de Biología Funcional y Genómica, University of Salamanca, Salamanca, Spain).

^bA. Bueno (Centro de Investigación del Cáncer, University of Salamanca, Salamanca, Spain).