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. 2014 Mar 27;9(3):e93208. doi: 10.1371/journal.pone.0093208

Figure 1. H-4073 inhibits STAT3 activation and head and neck cancer cell proliferation in a dose dependent manner.

Figure 1

A: HNSCC cell lines were treated with different concentrations of cisplatin (CDDP) and cell proliferation was assessed by MTT assay. B: Chemical structures of curcumin and H-4073. C: UM-SCC-74A cells were cultured in 10-cm culture dishes and treated with curcumin or H-4073 for 1 hour. Cellular uptake of curcumin and H-4073 was measured with a UV/Vis spectrophotometer. D: HNSCC cell lines were treated with different concentrations of H-4073 and cell proliferation was assessed by MTT assay. E: UM-SCC-74A cells were treated with different concentrations of H-4073 for 2 hours. STAT3, FAK, Akt and p38 phosphorylation was examined by Western blotting and equal protein loading was verified by stripping the blots and reprobing with GAPDH antibody.