Figure 5.

Time-dependent effect of Tet on the viability of primary cultured RGCs.

Notes: The relative cell numbers of primary cultured murine RGCs after 24 hours, 48 hours, 72 hours, 96 hours, and 120 hours exposure to Tet. Relative cell survival compared with DMSO in the 1 nM and 10 nM Tet conditions was 1.36±0.104 (P=0.009) and 1.56±0.105 (P=0.004) after 72 hours, respectively. After 96 hours, the relative cell survival in the 1 nM and 10 nM Tet conditions as compared to DMSO was 1.37±0.155 (P=0.011) and 1.41±0.085 (P=0.006), respectively. The differences between Tet, the control, and DMSO conditions were not significant at the 24 hour, 48 hour, or, 120 hour time points (P>0.05). Results are expressed as means ± SD. * or # P<0.05 as compared to DMSO control, one-way ANOVA (n=4).

Abbreviations: ANOVA, analysis of variance; DMSO, dimethyl sulfoxide; RGCs, retinal ganglion cells; SD, standard deviation; Tet, tetrandrine.