Table 3. Killing kinetics of cathelicidin-RC1 against E. coli ATCC25922.
Time | Colony Forming Units (×103, CFUs/ml) | ||||||||
0 min | 10 min | 20 min | 30 min | 45 min | 60 min | 90 min | 120 min | 180 min | |
Cathelicidin-RC1 | 83±7.9 | 79±21.2 | 40±28.1 | 50±10.1 | 8±5.6 | 0±0.0 | 0±0.0 | 0±0.0 | 0±0.0 |
Meropenem | 78±8.7 | 98±11.7 | 82±12.1 | 90±14.9 | 63±40.7 | 69±15.5 | 1±1.7 | 0.3±0.6 | 0±0.0 |
Control | 93±8.1 | 81±20.8 | 96±10.1 | 104±30.5 | 96±42.6 | 168±17.9 | 387±20.1 | 637±115.8 | 1104±145.7 |
E. coli ATCC25922 was mixed with samples at concentration of 5×MIC for 0, 10, 20, 30, 45, 60, 90, 120 and 160 mins. The results represent mean values of three independent experiments performed in duplicates. The MICs of cathelicidin-RC1 and meropenem against E. coli ATCC25922 are 4.69 and 0.01 μg/ml, respectively.