Table 5. Thermal tolerance of cathelicidin-RC1.

Temperature (°C)	MIC (μg/ml)
4	4.69
20	4.69
37	4.69
50	9.38
70	9.38
90	9.38

Thermal tolerance of cathelicidin-RC1 was determined by measuring MICs after incubation at different temperatures. Cathelicidin-RC1 solution (2 mg/ml, dissolved in sterile deionized water) was incubated at different temperatures (4, 20, 37, 50, 70, and 90°C) for 1 h, then the MICs of the peptides against E. coli ATCC25922 were determined. The results represent mean values of three independent experiments performed in duplicates.