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. 2014 Mar 27;10(3):e1004007. doi: 10.1371/journal.ppat.1004007

Figure 6. SV40 induces the B14-B12 complex to form foci on the ER membrane.

Figure 6

(A) B14 reorganizes into foci upon SV40 infection. CV-1 cells uninfected or infected with SV40 (MOI ≈30–50) for 16 h were fixed and stained with the indicated antibodies for immunofluorescence microscopy. (B) B12 reorganizes into foci upon SV40 infection. As in (A) except B12 antibodies were used. (C) VP1 is present in B14-B12 foci. As in (A) using B14 and VP1 antibodies. (D) As in (C) except using B12 antibodies. (E) Time course experiment monitoring foci formation. Cells were infected as in (A). Where indicated, BFA was added during entry. Cells were fixed at different time points after infection and stained with antibodies against BAP31, B14, or B12. Cells were scored positive if at least one focus was present in the cell. Values represent mean ± SD of three independent experiments. (F) Live cell imaging of GFP-B14 foci formation. Cells were transfected to express B14 with an N-terminal GFP tag. Cells were infected with SV40 and live cell imaging performed beginning at 2.5 h.p.i. Numbers in each frame indicate time in h.p.i.