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. 2014 Mar 27;10(3):e1004007. doi: 10.1371/journal.ppat.1004007

Figure 7. B14 requires its lumenal domain for SV40-induced foci formation.

Figure 7

(A) Transfected WT FLAG-B14 forms SV40-induced foci. Cells were transfected with empty vector or a plasmid expressing WT FLAG-B14. Cells were uninfected or infected with SV40 for 16 h, fixed, and stained with antibodies for immunofluorescence microscopy. (B) Δlumenal FLAG-B14 fails to reorganize into foci. Cells were transfected with WT or mutant FLAG-B14 and analyzed as in (A). (C) Quantification of (B). Fixed cells were scored for the presence or absence of FLAG-foci in cells expressing the respective FLAG-tagged protein. Values represent mean ± SD of three independent experiments. A statistically significant (p<0.05) difference was observed with Δlumenal FLAG-B14 when compared to all other versions of FLAG-B14. (D) BAP31 foci formation is not disrupted by expression of WT or mutant B14. As in (C) except cells were scored for BAP31 foci in cells expressing the indicated FLAG-tagged protein. Values represent mean ± SD of three independent experiments. No statistical differences were found, p>0.05.