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. 2014 Mar 27;9(3):e93287. doi: 10.1371/journal.pone.0093287

Figure 4. Effect of season on expression of candidate reference genes in immature oocytes from buffaloes.

Figure 4

Oocytes were retrieved from nulliparous (N; n = 12 and 11 oocytes, respectively) and multiparous (M; n = 11 and 13 oocytes, respectively) during winter (W) and summer (S). The amounts of ACTB (A), GAPDH (B), GUSB (C), HPRT1 (D), PPIA (E), RPL15 (F), SDHA (G), TBP (H) and YWHAZ (I) transcripts are expressed in relation to N during winter because this was considered as a reference group (see Material and methods for more information). P values for animal category, season and animal category*season are denoted in the insets above each graphic.