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. 2014 Mar 27;9(3):e93287. doi: 10.1371/journal.pone.0093287

Figure 6. Effect of reference gene selection on expression values of non-reference target genes in immature oocytes from buffaloes.

Figure 6

Oocytes were retrieved from nulliparous (N; n = 12 and 11 oocytes, respectively) and multiparous (M; n = 11 and 13 oocytes, respectively) during winter (W) and summer (S). The amounts of HSPA1A plus HSPA1B (termed in the current work as HSPA1AB; A–D) and HSP90AA1 (E–H) transcripts are expressed in relation to N during winter because this was considered as a reference group (see Material and methods for more information). Expression values were normalized either by TBP (A and E), YWHAZ (B and F), geometric mean of YWHAZ, GUSB and GAPDH (C and G) or not normalized (D and H). P values for animal category, season and animal category*season are denoted in the insets above each graphic.