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. 2014 Mar 27;9(3):e93452. doi: 10.1371/journal.pone.0093452

Figure 2. RACE strategies for cloning Ovocleidin-17 and nucleotide and deduced amino acid sequence of OC-17.

Figure 2

The protocol of the SuperScriptTMIII RT Kit (Invitrogen Life Technologies Bio Inc.) was followed. Gene RACE Primers (GRP) anneal to the adaptor sequence, and Gene Special Primers (GSP) were designed to be complementary to the Ovocleidin-17 cDNA. The start and stop codons of the open reading frame are marked in red color. Black box indicates the poly-adenylation signal (AATAAA). The nucleotides marked with red color were obtained by RACE. Brown box represented the extra 19 deduced amino acids compared with published OC-17 amino acids (Q9PRS8), which is a putative signal peptide. Note that there is a one nucleotide difference (green color with black underline) between the RACE cDNA and the assembled transcript.