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. 2014 Mar 27;9(3):e93452. doi: 10.1371/journal.pone.0093452

Table 1. Primers for amplifying cDNA and quantifying mRNA of OC17.

Primer category Primer name Nucleotide sequences Size (bp)
RACE primers 5′GRP 5′-CGACTGGAGCACGAGGACACTGA-3′ 212
5′GSP 5′-GAGCTCCCGGCTGAAGAAGC-3′
3′GRP 5′-GCTGTCAACGATACGCTACGTAACG-3′ 235
3′GSP 5′-GAGGAGGCCTTCACCTCGTG-3′
SP-F 5′-GGCTGCGTTCTGCTGCTC-3′ 481
SP-R 5′-TTGTTGTGTTGTTGTCCATTCA-3′
5′Oligo 5′-CGACTGGAGCACGAGGACACTGACATGGACTGAAGGAGTAGAAA-3′ 44
3′Oligo 5′-GCTGTCAACGATACGCTACGTAACGGCATGACAGTGTTTTTTTTTTTTTTTTTTTTTTTT-3′ 60
qRT-PCR primers P-OC-17-F 5′-CGTTCTGCCGCCGTTGGG-3′ 96
P-OC-17-R 5′-CCCGCGACGCGTTGAGGA-3′
P-β-Actin -F 5′-TATGTGCAAGGCCGGTTTC-3′ 110
P-β-Actin -R 5′-TGTCTTTCTGGCCCATACCAA-3′

GRP means Gene RACE Primer which corresponded to the adaptor sequence and GSP means Gene Special Primer which was complementary to the OC17 cDNA. Primers for each sequence end are indicated either 5′ or 3′. Forward primers and reverse primers for qRT-PCR have prefixes ‘F’ and ‘R’, respectively. 5′GRP and 5′GSP were used for the cloning of 5′ end of the OC17 cDNA while 3′GRP and 3′GSP were used for the 3′ end. SP-F and SP-R were used for validation of the other parts of the OC17 cDNA. 5′Oligo and 3′Oligo were provided by the Gene Racer Kit.