Figure 6. Five novel activators of HitRS.

B.anthracis Sterne (WT) and ΔhitRS were transformed with the xylE reporter plasmid, phit. The strains were treated with either 50 μM ‘205 or a range of concentrations of the following compounds: (A) nordihydroguaiaretic acid (NDGA), (B) chlorpromazine, (C) targocil, (D) vancomycin, and (E) sodium phosphate (NaH₂PO₄) pH 7. Cultures were grown for 6 h and harvested. Cells were lysed and XylE activity was quantified. In all instances, error bars represent ±SEM of data averaged from two independent experiments performed with biological triplicates. Significance was calculated by an unpaired Student's t-test, where *  =  P≤0.01, **  =  P≤0.001, and ***  =  P≤0.0001.