Table 1. List of mutants selected using the per-pool screening method.
| MUTANT LACKING | ARCI† ± SE IN | ||||||
| ORF(s) | GENE(s) | CATEGORY OF THE GENE | LYMPH NODE | SPLEEN | |||
| Metabolism | YPO2303-YPO2302 | pntAB | Nicotinate and nicotinamide metabolism | 0.0 | ±0 | 0.0 | ±0 |
| YPO2288 | amn | Purine metabolism | 0.0 | ±0 | 0.0 | ±0 | |
| YPO1133 | gpmA | Glycolysis/Gluconeogenesis | 0.0 | ±0 | 0.0 | ±0 | |
| YPO2269 | bioD2 | Biotin metabolism | 2.6 | ±2 | 12.6 | ±10 | |
| YPO1139-1136 | galETKM | Galactose metabolism | 3.8 | ±2 | 10.2 | ±10 | |
| YPO3826-YPO3824 | glpABC | Glycerophospholipid metabolism | 5.4 | ±5 | 0.8 | ±0 | |
| YPO1514-YPO1512 | cdd yohJK | Pyrimidine metabolism | 7.1 | ±5 | 31.9 | ±14 | |
| YPO3953-YPO3955 | gntKT y3874 gntR | Pentose phosphate pathway | 11.7 | ±9 | 7.9 | ±5 | |
| YPO2734-YPO2743 | ccmABCDEFGH1H2 vacJ | Cytochrome c-type biogenesis | 12.0 | ±10 | 7.7 | ±3 | |
| YPO0579-YPO0581 | uxaC uxuBA | Pentose and glucuronate interconversions | 14.4 | ±10 | 17.4 | ±10 | |
| YPO3808-YPO3804 | livKHMGF | Amino-acids Tansport | 14.7 | ±10 | 16.2 | ±10 | |
| YPO3719 | lysC | Amino-acids metabolism | 18.2 | ±11 | 15.1 | ±10 | |
| YPO2325 | dalD | Pentose and glucuronate interconversions | 19.1 | ±11 | 13.1 | ±10 | |
| YPO0989-YPO0994 | iucABCD iutA | Iron transport | 20.2 | ±13 | 10.1 | ±10 | |
| YPO2965-YPO2969 | dmsABC | Respiration | 30.5 | ±14 | 18.3 | ±11 | |
| Attachment | YPO0698-YPO0700 | papCD gafB | Attachment/colonization | 0.3 | ±0 | 0.0 | ±0 |
| YPO2905 | ail | Attachment/colonization | 8.3 | ±5 | 14.2 | ±8 | |
| Stress response | YPO2714 | rseC | Regulation | 0.5 | ±0 | 0.9 | ±1 |
| YPO4085 | ibpA | Molecular chaperone | 0.9 | ±0 | 1.6 | ±1 | |
| YPO1359 | hcr | NADH-oxydoreductase | 14.9 | ±11 | 21.2 | ±11 | |
| YPO2958-YPO2960 | yfuABC | Stress response | 23.0 | ±13 | 17.9 | ±10 | |
| YPO0049 | radC | Stress response | 29.2 | ±14 | 18.1 | ±11 | |
| Uncharacterized | YPO2586-YPO2587 | - | Inositol phosphate metabolism | 0.0 | ±0 | 0.0 | ±0 |
| YPO0618-YPO0617 | - | - | 0.1 | ±0 | 0.1 | ±0 | |
| YPMT1,66c | - | - | 0.5 | ±0 | 1.2 | ±1 | |
| YPO3798-YPO3801 | - | Attachment/colonization | 1.1 | ±1 | 1.1 | ±1 | |
| YPO2561-YPO2560 | - | - | 4.6 | ±5 | 1.4 | ±1 | |
| YPO1992 | - | - | 8.6 | ±6 | 15.0 | ±10 | |
| YPO0337 | - | - | 9.5 | ±9 | 5.4 | ±5 | |
| YPO0656 | yqiC | - | 12.5 | ±13 | 0.0 | ±0 | |
| YPO3369 | - | - | 22.0 | ±13 | 4.4 | ±2 | |
| YpcD1.23 | - | - | 26.2 | ±12 | 10.9 | ±5 | |
| YPO0841-YPO0843 | ydeMN - | - | 11.5 | ±9 | 12.5 | ±10 | |
| YPO3008-YPO3009 | - | Regulation | 13.6 | ±10 | 12.6 | ±10 | |
| YPO3991 | yhjJ | - | 14.5 | ±10 | 18.8 | ±11 | |
| YPO1307 | - | - | 15.2 | ±9 | 14.0 | ±7 | |
| YPO1380 | ycaD | Transport | 15.4 | ±12 | 16.0 | ±12 | |
| YPO3342-YPO3343 | yhjA - | - | 15.5 | ±10 | 15.9 | ±10 | |
| YPO1289-YPO1287 | - | Amino-acids metabolism | 15.6 | ±9 | 32.7 | ±15 | |
| YPO2337 | - | Regulation | 16.7 | ±10 | 25.4 | ±11 | |
| YPO3025 | - | - | 17.8 | ±10 | 39.1 | ±14 | |
| YPO2745 | - | - | 18.9 | ±11 | 37.1 | ±15 | |
| YPO0585 | - | - | 19.3 | ±11 | 27.6 | ±14 | |
| y4094 | - | - | 20.5 | ±11 | 17.3 | ±10 | |
| YPO2006-YPO2008 | - | - | 20.7 | ±13 | 18.5 | ±12 | |
| YPO1862 | - | - | 25.4 | ±13 | 16.7 | ±12 | |
| YPO2282 | - | - | 31.4 | ±13 | 15.4 | ±11 | |
| YPO0856-YPO0852 | - | Transport & Galactose metabolism | 32.1 | ±15 | 14.3 | ±10 | |
†
, data were obtained from groups of 10 rats inoculated intradermally with a pool of 5 mutants (with 20 CFU of each mutant).