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. Author manuscript; available in PMC: 2015 Mar 26.
Published in final edited form as: Chem Rev. 2014 Jan 8;114(6):3224–3265. doi: 10.1021/cr400496q

Figure 2. Photophysical properties of fluorophores.

Figure 2

(A) Simplified Jablonski diagram representing excitation (Ex), fluorescence (Fl) emission, internal conversion (IC), vibrational relaxation (VR), non-radiative decay (NR), intersystem crossing (ISC), phosphorescence (Ph) and photobleaching (PB), and the respective timescales at which these processes occur. S0, singlet ground state; S1, singlet excited state; T1, triplet state. (B) A simulated intensity trajectory of a single molecule with two blinking events (orange arrow) and a single photobleaching step (dotted grey arrow).