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. 2013 Nov 26;101(1):87–96. doi: 10.1093/cvr/cvt232

Figure 4.

Figure 4

Effects of Tollip overexpression on cardiac hypertrophy. (A) Schematic diagram of the αMHC-Tollip transgene construct. (B) Representative western blots of the human Tollip protein in heart tissues from four TG lines and WT mice (n = 6). (C) Representative western blots of the human Tollip protein in various tissues from the TG mice (n = 4), as indicated. (D) Gross hearts and HE and WGA-FITC staining were performed at 8 weeks after Sham or AB surgery (n = 6). Statistical results for (E) the myocyte cross-sectional areas (n = 100+ cells), (F) HW/BW, (G) LW/BW, and (H) HW/TL of the indicated groups (n = 11–13). (I) Expression of hypertrophic markers was determined by real-time RT–PCR in NTG and TG mice (n = 4). (J) PSR staining on histological sections of the LV was performed on the indicated groups 8 weeks after AB (n = 6). (K) Fibrosis areas from the histological sections were quantified using an image analysis system (n = 26–28 fields). (L) Real-time RT–PCR was performed to determine mRNA levels of CTGF, collagen Iα, and collagen III in the hearts of the indicated mice (n = 4). *P < 0.05 vs. NTG /sham; #P < 0.05 vs. NTG/AB.