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. 2014 Mar 12;6(3):1049–1061. doi: 10.3390/toxins6031049

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© 2014 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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SDS-PAGE analyses of wild type NetB and variants of NetB. After protein purification, wild type NetB and NetB variants were analysed by SDS-PAGE. Lane 1: protein marker (molecular mass is indicated in kDa to the left); lane 2: wild type NetB; lane 3: NetB K41H; lane 4: NetB K71A; lane 5: NetB P155A; lane 6: NetB D156C; lane 7: NetB D250A.